BMP-2 and bFGF in an irradiated bone model

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Journal Article

Publication Details

Interim status: Citation only.

Springer, I. N. G., Niehoff, P., Açil, Y., Marget, M., Lange, A., Warnke, P. H., et al. (2008). BMP-2 and bFGF in an irradiated bone model. Journal of cranio-maxillofacial surgery, 36(4), 210-217.

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2008 HERDC submission. FoR Code: 1115

© Copyright European Association for Cranio-Maxillofacial Surgery, 2007


Basic fibroblast growth factor (bFGF) is considered to enhance angiogenesis and to support bone formation in the presence of vital bone cells. Bone morphogenetic protein-2 (rhBMP-2) is known to induce bone formation. The aim of this study was to analyze the effect of bFGF and rhBMP-2 in the irradiated mandible.

Material and methods
The right mandibles of 24 rats were irradiated with a single dose of 20 Gy at a high-dose-rate (HDR) after loading machine (bio effective equivalent dose to ca. 45×2Gy). After 12 weeks 100μg rhBMP-2 (n=6 animals, group 1), 100μg bFGF (n=6 animals, group 2) and 100μg rhBMP-2 plus 100μg bFGF (n=6 animals, group 3) were injected along the right mandible (left mandible: no irradiation, no growth factor). Another 6 animals (group 4) remained untreated after the irradiation. After another 7 weeks the specimens were examined by non-decalcified histology.

Bone apposition of the experimental versus control sides was not statistically significantly different when one of the growth factors was applied alone (rhBMP-2: p=0.917; bFGF: p=0.345). Average bone apposition was significantly decreased on the experimental sides of group 3 (rhBMP-2+bFGF: p=0.046) and group 4 (p=0.008). Average bone densities were unaffected in all settings (for all p>0.1).

The application of bFGF and the application of rhBMP-2 alone did result in predictable bone generation in the irradiated mandible with the bone apposition being equal to that of the non-irradiated side. The application of both growth factors together or none at all after irradiation results in significantly reduced bone apposition.

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