Bioactive coating of titanium surfaces with recombinant human ß-defensin-2

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Pfeufer, N. Y., Hofmann-Peiker, K., Muhle, M., Warnke, P. H., et al. (2011). Bioactive coating of titanium surfaces with recombinant human ß-defensin-2 (rHußD2) may prevent bacterial colonization in orthopaedic surgery. Journal of Bone and Joint Surgery-American Volume, 93 (9), 840-846.

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2011 HERDC submission. FoR code: 090300, 110300

© Copyright by The Journal of Bone and Joint Surgery Incorporated, 2011




A promising strategy to prevent infections around orthopaedic titanium implants is to use naturally occurring cationic antimicrobial peptides (CAMPs) such as the human ß-defensin-2 as antibacterial coatings. Human antimicrobial peptides represent a part of the innate immune system and have a broad antimicrobial spectrum against bacteria, fungi, and viruses.

In the present study, titanium surfaces were functionalized by four different self-assembled monolayers (SAMs) forming methoxy silanes: (1) hexadecyltrimethoxysilane, (2) dimethoxymethyloctylsilane, (3) allyltrimethylsilane, and (4) 3-aminopropyltrimethoxysilane. In addition, calf skin type-I collagen was cross-linked to the SAM surface 3- aminopropyltrimethoxysilane by means of two different treatments: (1) N-hydroxysuccinimide and (2) glutaraldehyde. The functionalized titanium surfaces were coated with recombinant human ß-defensin-2 (rHußD2), an antimicrobial peptide, and were tested for antibacterial activity against Escherichia coli. The release of rHußD2 was quantified by means of enzyme-linked immunosorbent assay (ELISA).

The coating of functionalized titanium surfaces with rHußD2 was successful. Recombinant HußD2 was eluted from the titanium surfaces continuously, yielding antimicrobial activity up to several hours.

Natural antibiotics such as rHupD2 integrated into the metal surface of titanium implants may be a promising tool to prevent and control infections around orthopaedic implants.

Clinical Relevance:
This kind of titanium surface modification may provide an alternative treatment of serious, life threatening infections related to prosthetic implant surgery.

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This document has been peer reviewed.