Novel ceramic bone replacement material CeraBall® seeded with human mesenchymal stem cells

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Journal Article

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Interim status: Citation only.

Douglas, T., Liu, Q., Humpe, A., Wiltfang, J., Sivananthan, S., & Warnke, P. H. (2010). Novel ceramic bone replacement material CeraBall® seeded with human mesenchymal stem cells, Clinical Oral Implants Research, 21(3), 262-267.

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2010 HERDC submission. FoR Code: 100404, 110504

© Copyright 2009 John Wiley & Sons A/S


Objectives: Hydroxyapatite (HA) and tricalcium phosphate (TCP) are two very common ceramic materials for bone replacement. A recently developed material for bone replacement is CeraBall®, which is a mixed HA-TCP scaffold available as porous spherical scaffolds of diameter 4 and 6 mm. Before their use as bone replacement materials in vivo, in vitro testing of these scaffolds is necessary. The goal of this study was to characterise 4 and 6 mm CeraBall® scaffolds in vitro with a view to their future use as bone replacement materials.

Materials and methods: The proliferation of human mesenchymal stromal cells (hMSCs) seeded on CeraBall® scaffolds was evaluated quantitatively using the WST [Water soluble tetrazolium ((4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1, 3-benzene disulfonate)] test and qualitatively by scanning electron microscopy (SEM). In addition, the standard MTT [(3-(4, 5-Dimenthylthiazol-2-Y1)-2, 5-Diphenyltetrazolium bromide)] biocompatibility test and cell vitality staining were performed using hMSCs. CeraBall® scaffolds were also tested for their mechanical properties.

Results: SEM and WST test results showed that hMSCs proliferated on CeraBall® scaffolds over the course of 9 days. Proliferation was similar to that seen on tissue culture polystyrene (control). Cells showed a well-spread morphology and formed 'sheets' on the surface of scaffolds. Invasion of pores was observed. Good biocompatibility was demonstrated by MTT test results and cell vitality staining. Scaffolds of both 4 and 6 mm were able to withstand compressive loads of 5 N.

Conclusions: CeraBall® scaffolds show good biocompatibility in vitro for hMSCs. This opens the way for in vivo applications.

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