Highly degraded biological samples may be encountered in cases of mass disasters, missing persons cases, and forensic case work. As biological tissue degrades, DNA becomes progressively more fragmented and a decrease in the ability to gain an accurate DNA profile result. The successful typing of highly degraded DNA can be further complicated by also having chemical modifications (DNA damage). Such highly degraded samples often produce incomplete or no STR profiles. Alternate genotyping strategies such as mini-STRs and SNPs have proven to be more successful in profiling such difficult samples and were therefore investigated in this thesis. In an attempt to improve the quality of highly degraded DNA template prior to multiple displacement amplification (MDA) and STR-typing, several repair methods were investigated.
Year Manuscript Completed
Sociology, Criminology and Penology (0627)
DNA damage; DNA damage Research Methodology; DNA Analysis; Forensic biology
Primary Language of Manuscript
Recommended CitationSheree R. Hughes-Stamm (2012) DNA Typing Methods for Highly Degraded Samples, PhD, ePublications@bond, Faculty of Health Sciences and Medicine.
01Front.pdf (803 kB)