Title

Toxicity of industrially relevant chlorinated organic solvents in vitro

Date of this Version

1-1-2013

Document Type

Journal Article

Publication Details

Citation only

McDermott, C., & Heffron, J. J. A. (2013). Toxicity of industrially relevant chlorinated organic solvents in vitro. International Journal of Toxicology, 32(2), 136-145.

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© Copyright,Sage Publications , 2013

2013 HERDC Submission. FoR code: 110312

ISSN

1091-5818

Abstract

The cytotoxic effects of 4 industrially important chlorinated organic solvents, dichloromethane (DCM), 1,2-dichloroethane (DCE), trichloroethylene (TCE), and tetrachloroethylene (PERC) in vitro, were investigated. Jurkat T cells were exposed to the solvents individually for 72 hours and changes in reactive oxygen species (ROS) formation, cell proliferation, intracellular free calcium concentration ([Ca2+]), and caspase-3 activity were measured. There was a concentration-dependent increase in the ROS formation and intracellular free [Ca2+] following exposure to each of the solvents. This was accompanied by a decrease in the cell proliferation. Solvent potency decreased in the following order: PERC > TCE > DCM > DCE. Caspase-3 activity was increased in a concentration-dependent manner by TCE and PERC but was not significantly altered by DCM or DCE. n-Acetyl-l-cysteine pretreatment showed that changes in the intracellular free [Ca2+] and caspase-3 activity were independent of ROS formation. However, increased ROS formation did play a causal role in the decreased cell proliferation observed.

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This document has been peer reviewed.