Title

High-throughput sequencing of plasma MicroRNA in chronic fatigue syndrome/myalgic encephalomyelitis

Date of this Version

2014

Document Type

Journal Article

Publication Details

Citation only

Brenu, E. W., Ashton, K. J., Batovska, J., Staines, D. R., & Marshall-Gradisnik, S. M. (2014). High-throughput sequencing of plasma MicroRNA in chronic fatigue syndrome/myalgic encephalomyelitis. PLoS ONE, 9(9).

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© Copyright, The Authors, 2014

This work is licensed under a Creative Commons License 4.0

ISSN

1932-6203

Abstract

Background: MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME. Results: Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients. Conclusion: Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers

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