Title

Bioactive coating of titanium surfaces with recombinant human ß-defensin-2

Date of this Version

5-4-2011

Document Type

Journal Article

Publication Details

Citation only

Pfeufer, N. Y., Hofmann-Peiker, K., Muhle, M., Warnke, P. H., et al. (2011). Bioactive coating of titanium surfaces with recombinant human ß-defensin-2 (rHußD2) may prevent bacterial colonization in orthopaedic surgery. Journal of Bone and Joint Surgery-American Volume, 93 (9), 840-846.

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2011 HERDC submission. FoR code: 090300, 110300

© Copyright by The Journal of Bone and Joint Surgery Incorporated, 2011

ISSN

0021-9355

Abstract

Background:
A promising strategy to prevent infections around orthopaedic titanium implants is to use naturally occurring cationic antimicrobial peptides (CAMPs) such as the human ß-defensin-2 as antibacterial coatings. Human antimicrobial peptides represent a part of the innate immune system and have a broad antimicrobial spectrum against bacteria, fungi, and viruses.

Methods:
In the present study, titanium surfaces were functionalized by four different self-assembled monolayers (SAMs) forming methoxy silanes: (1) hexadecyltrimethoxysilane, (2) dimethoxymethyloctylsilane, (3) allyltrimethylsilane, and (4) 3-aminopropyltrimethoxysilane. In addition, calf skin type-I collagen was cross-linked to the SAM surface 3- aminopropyltrimethoxysilane by means of two different treatments: (1) N-hydroxysuccinimide and (2) glutaraldehyde. The functionalized titanium surfaces were coated with recombinant human ß-defensin-2 (rHußD2), an antimicrobial peptide, and were tested for antibacterial activity against Escherichia coli. The release of rHußD2 was quantified by means of enzyme-linked immunosorbent assay (ELISA).

Results:
The coating of functionalized titanium surfaces with rHußD2 was successful. Recombinant HußD2 was eluted from the titanium surfaces continuously, yielding antimicrobial activity up to several hours.

Conclusions:
Natural antibiotics such as rHupD2 integrated into the metal surface of titanium implants may be a promising tool to prevent and control infections around orthopaedic implants.

Clinical Relevance:
This kind of titanium surface modification may provide an alternative treatment of serious, life threatening infections related to prosthetic implant surgery.

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This document has been peer reviewed.