Title
Endocultivation: The influence of delayed vs. simultaneous application of BMP-2 onto individually formed hydroxyapatite matrices for heterotopic bone induction
Date of this Version
5-31-2012
Document Type
Journal Article
Abstract
When bone morphogenetic protein (BMP) is delivered to matrices in vivo may affect tissue engineered bone constructs for jaw reconstruction after cancer surgery. This study compared the effects of BMP application at different times after matrix implantation for heterotopic bone induction in a rat model. Hydroxyapatite blocks were implanted unilaterally onto the surface of the latissimus dorsi muscle. A second block was implanted onto the contralateral muscle after 1, 2 or 4 weeks and 200 μg rhBMP-2 was injected into the blocks on both sides. Bone formation and density inside the blocks was analysed by CT and histology. 8 weeks after BMP application increases in bone density within the scaffolds were most pronounced in the simultaneous application group (179 HU). Less pronounced increases were observed for the 1 (65 HU), 2 (58 HU) and 4 (31 HU; p < 0.0001) week delay group. Homogeneous bone induction started from the central channel of the blocks. Capillaries and larger vessels were seen in all constructs, samples receiving delayed BMP treatment demonstrated significantly greater neovascularization. Delayed application of BMP was less effective for heterotopic bone formation than simultaneous application. A central channel allows homogeneous bone induction directly from the centre of the blocks.
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Publication Details
Citation only.
Becker, S. T., Bolte, H., Schünemann, K., Seitz, H., Bara, J. J., Beck-Broichsitter, B. E., Russo, P. A. J., Wiltfang, J., & Warnke, P. H. (2012). Endocultivation: The influence of delayed vs. simultaneous application of BMP-2 onto individually formed hydroxyapatite matrices for heterotopic bone induction. International journal of oral and maxillofacial surgery, In Press published online, 1-8. http://dx.doi.org/10.1016/j.ijom.2012.03.031
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© Copyright International Association of Oral and Maxillofacial Surgeons, 2012. Published by Elsevier Ltd. All rights reserved.